Abstract:
Consumption of vegetables contaminated with pathogenic micro organisms, particularly, in areas where urban waste
water is used to irrigate vegetable crops is suspected to be a factor in outbreaks of some public health diseases. The
microbiological quality of lettuce on three farms where waste water is used in the Kumasi Metropolis in Ghana was
therefore studied for their Total colifoms, faecal coliforms, enterococci, E. coli and Salmonella CFU levels using
standard methods. Total coliforms on the lettuce varied from 4.93×104CFU to 6.17×104CFU. Faecal coliforms
ranged from 3.48×103CFU to 4.66×104CFU and E. coli 2.98×103CFU to 3.86×104CFU. Samonella and enterococci
levels ranged from 2.50×102CFU to 2.72×102CFU and 0.68×100CFU to 2.05×100CFU respectively. In most cases the
highest bacterial contamination was associated with lettuce grown at Atonsu and the lowest was at Karikari farms
located within the Kumasi Metropolis. The differences in total colifom counts at the two locations were significant (P
< 0.001). Differences in bacteria counts for faecal colifoms (P < 0.000) and E. coli (P < 0.000) were significantly
higher than counts associated with the other bacteria from all the three farms. In general, bacterial counts on farm
lettuce exceeded the recommended World Health Organization (WHO) and International Commission on
Microbiological Specifications for Food (ICMSF) standards of 103
. Wastewater use on farms therefore, could be the
main contributor to lettuce contamination and outbreak of communicable diseases. Education on use of effective
de-contamination methods before eating will help reduce the risk associated with the consumption of such
contaminated vegetables.
Key Words: lettuce, contamination, bacteria, Kumasi, Ghana.
